import pandas as pd
import numpy as np

path = r"D:\excelData\data.xlsx"

x1 = np.random.randn(100, 2)
df1 = pd.DataFrame(x1)

x2 = np.random.randn(100, 2)
df2 = pd.DataFrame(x2)

writer = pd.ExcelWriter(path, engine = 'xlsxwriter')
df1.to_excel(writer, sheet_name = 'x1')
df2.to_excel(writer, sheet_name = 'x2')
writer.save()
writer.close()

或者

import pandas as pd
import numpy as np
from openpyxl import load_workbook
path = r"D:\excelData\data.xlsx"
book = load_workbook(path)
writer = pd.ExcelWriter(path, engine = 'openpyxl')
writer.book = book
x3 = np.random.randn(100, 2)
df3 = pd.DataFrame(x3)
x4 = np.random.randn(100, 2)
df4 = pd.DataFrame(x4)
df3.to_excel(writer, sheet_name = 'x3')
df4.to_excel(writer, sheet_name = 'x4')
writer.save()
writer.close()

或者

import pandas as pd
from openpyxl import load_workbook
from openpyxl.utils.dataframe import dataframe_to_rows

fileLocation =r'D:\excelData\data.xlsx'
writeLocation=r'D:\excelData\dataNew.xlsx'
data = {'Name':['C','Java','Python'],'Age':[32,43,34],'Salary':[20000,34000,32000]}
df = pd.DataFrame(data)
book = load_workbook(fileLocation)
sheet = book.create_sheet("Sheet Name")
for row in dataframe_to_rows(df, index=False, header=True):
    sheet.append(row)
book.save(writeLocation)